134 135 animals 136 Search Results


c neoformans 136 c neoformans 52d  (ATCC)
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ATCC c neoformans 136 c neoformans 52d
C Neoformans 136 C Neoformans 52d, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epcam conjugated antibody  (Miltenyi Biotec)
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Miltenyi Biotec epcam conjugated antibody
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Epcam Conjugated Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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animals 138  (Jackson Laboratory)
86
Jackson Laboratory animals 138
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Animals 138, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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134 135 animals 136  (Jackson Laboratory)
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Jackson Laboratory 134 135 animals 136
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
134 135 Animals 136, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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animal treatment 135 male dahl ss rats  (Charles River Laboratories)
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Charles River Laboratories animal treatment 135 male dahl ss rats
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Animal Treatment 135 Male Dahl Ss Rats, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rheometer uds 200  (Anton Paar)
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Anton Paar rheometer uds 200
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Rheometer Uds 200, supplied by Anton Paar, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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convection oven despatch model lxd1-42-2  (Despatch Industries)
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Despatch Industries convection oven despatch model lxd1-42-2
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Convection Oven Despatch Model Lxd1 42 2, supplied by Despatch Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd45  (Miltenyi Biotec)
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Miltenyi Biotec cd45
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Cd45, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crispr cas9 134 135 136 antibodies  (Proteintech)
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Proteintech crispr cas9 134 135 136 antibodies
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Crispr Cas9 134 135 136 Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bca protein assay kit  (Thermo Fisher)
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Thermo Fisher bca protein assay kit
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diazepam dzp  (Anwendung GmbH)
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Anwendung GmbH diazepam dzp
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Diazepam Dzp, supplied by Anwendung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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housing 138 c57bl 6nj mice  (Jackson Laboratory)
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Jackson Laboratory housing 138 c57bl 6nj mice
Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: <t>EPCAM</t> (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Housing 138 C57bl 6nj Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: EPCAM (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Frontiers in Toxicology

Article Title: A New Immortalized Human Alveolar Epithelial Cell Model to Study Lung Injury and Toxicity on a Breathing Lung-On-Chip System

doi: 10.3389/ftox.2022.840606

Figure Lengend Snippet: Expression of distinct alveolar epithelial cell markers in AX iAECs cultured on AX Lung-on-chip. (A) Timeline and schematic of the AX iAECs cultured on the AX12. (B) Representative immunofluorescent staining for AX iAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: EPCAM (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AX iAECs cultured on AX Lung-on-chip (N = 2; n = 4) for 7 days. (E) AX iAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AX iAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Mono-culture staining was performed with: mouse anti-ZO-1 antibody (C# 33-9100, Fisher T Scientific), rabbit anti-mature SP-C antibody (C# WRAB- 76694, Seven Hills Bioreagents), mouse anti-HTI-56 (C# TB-29AHTI-56, Terrace Biotech), rabbit anti-MUC1 (C#NBP1-60046, Novus Biologicals), Epcam conjugated antibody (C# 130-111-117, Milteny Biotec), rabbit anti-HOPX (C# ab106251, Abcam), rabbit anti-ABCA3 (C# ab99856, Abcam), mouse anti-PECAM1 (#3528 Cell Signaling).

Techniques: Expressing, Cell Culture, Staining, Gene Expression